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Sample Discontinued
Experience: None
Not Used
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Part:BBa_K382013:Design

Designed by: Christina Agapakis   Group: iGEM10_Harvard   (2010-10-26)


amiRNA Construct for Arabidopsis GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 418
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 418
    Illegal NotI site found at 425
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 418
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 418
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed microRNA sequences with Web microRNA Designer http://wmd3.weigelworld.org/cgi-bin/webapp.cgi?page=Designer;project=stdwmd


Source

Modified RS300 vector http://wmd3.weigelworld.org/cgi-bin/webapp.cgi?page=Home;project=stdwmd

References